Lateral flow immunoassay based on dual spectral-overlapped fluorescence quenching of polydopamine nanospheres for sensitive detection of sulfamethazine

作者：Wang, Zexiang; Xing, Keyu; Ding, Nengshui; Wang, Suhua; Zhang, Ganggang^*; Lai, Weihua^*

来源：Journal of Hazardous Materials, 2022, 423: 127204.

DOI：10.1016/j.jhazmat.2021.127204

• Lateral flow immunoassay
• Polydopamine nanospheres
• Dual spectral-overlapped fluorescence quenching
• Sulfamethazine
• Chicken

Herein, we propose a lateral flow immunoassay (LFIA) based on the dual spectral-overlapped fluorescence quenching of polydopamine nanospheres (PDANs) caused by the inner filter effect to sensitively detect sulfa-methazine (SMZ). The fluorescence quenching LFIA device consists of four parts: absorbent pad, polyvinyl chloride pad, sample pad, and nitrocellulose membrane. Compared with traditional quenchers such as gold nanoparticles (AuNPs) with single spectral-overlapped quenching ability, PDANs can quench the excitation light and emission light of three fluorescence donors (aggregation-induced emission fluorescent microsphere, AIEFM; fluorescent microsphere, FM; and quantum dot bead, QB). The fluorescence intensity changes (Delta F) are numer-ically larger for PDANs-LFIA (Delta F-AIEFM = 2315, Delta F-FM = 979, Delta F-QB = 910) than those for AuNPs-LFIA (Delta AF(AIEFM) = 1722, Delta F-FM = 833, Delta F-QB =;520). AIEFM-based PDANs-LFIA exhibits a large AF (2315) in response to the changes in the SMZ concentration, and produces a high signal-to-noise ratio. The limit of detection (LOD) and visual LOD of LFIA based on PDANs quenching AIEFM for the detection of SMZ in chicken are 0.043 and 0.5 ng/mL, respectively. The results confirm that the proposed method can be used for the detection of hazardous materials in practical applications.

• 单位
  江西农业大学; 南昌大学