Micro-RNA Regulation on Oct4 Gene Expression and Functional Differentiation in Skin Cancer Cells

作者：Li, Yuan; Dai, Yongjiang; Bai, Shun; Yang, Bin^*

来源：Journal of Biomaterials and Tissue Engineering, 2021, 11(11): 2210-2215.

DOI：10.1166/jbt.2021.2805

• Mir-335
• Skin Cancer
• Oct4
• Cell Mobility
• Cell Infiltration
• Gene Regulation

Micro-RNA has a very important role in gene regulation. The stem gene Oct4 is related with the growth, mobility, and infiltration of skin cancer cells. Studying the regulatory mechanism of microRNAs of Oct4 in skin cancer cells is of important clinical significance. Oct4 gene was analyzed using bioinformatics methods to find mir-RNA with regulatory functions. mir-RNA high-expression vector and suppression vector with pcDNA3.1-EGFP was transfected to skin cancer cell line HS-4 followed analysis of Oct4 expression 24 h and 48 h after transfection and transwell in-vitro cell invasion assay. Bioinformatics showed that mir-335 has relationship with the Oct4 gene. pcDNA3.1-EGFP-335-up and pcDNA3.1-EGFP-335-down were successfully constructed. 24 and 48 hours after transfection, the Oct4 expression in the high-expression group was gradually and significantly decreased (P < 0.05). Meanwhile, the cell migration and infiltration capacity was decreased significantly and showed time dependence with significant differences between groups (P < 0.05). mir-335 expression in suppression group was reduced without change of Oct4 (P > 0.05). Increased mir-335 can decrease the performance of Oct4 in skin cancer cells and inhibit the infiltration ability of cells without affecting cell infiltration capability.

• 单位
  海南医学院; 1; 南方医科大学