YAP signaling is involved in WDR1-regulated proliferation and migration of non-small-cell lung cancer cells

作者：An, Ran; Wang, Junyan; Chen, Xuan; Xu, Ruifeng; Hu, Jisheng; Liu, Zhongying; Wei, Chanjuan; Zhang, Chenxi^*; Yuan, Baiyin^*

来源：Experimental Biology and Medicine, 2022, 247(18): 1619-1629.

DOI：10.1177/15353702221110645

• WDR1
• YAP
• non-small-cell lung cancer
• cytoskeleton

As a major co-factor of F-actin depolymerization, WD-repeat domain 1 (WDR1) affects the cellular microenvironment by cytoskeleton remodeling, thereby influencing cell molecular behavior. Our previous study showed that WDR1 activates YAP (Yes-associated protein) signaling in non-small-cell lung cancer (NSCLC) cells, but the mechanism remains unclear. We discovered that knockdown WDR1 in NSCLC cells decreased the expression of YAP and the nucleus-to-cytoplasm ratio. Disruption of cortical stress by drugs significantly inhibited YAP nuclear trafficking and enhanced YAP phosphorylation. In WDR1-knockdown NSCLC cells, inhibition of Hippo pathway reduced the nuclear exclusion of YAP and phosphorylated YAP. Our data suggest that WDR1-mediated cortical stress might be involved in regulating YAP signaling, thereby affecting the proliferation and migration of NSCLC cells.