Detection of biological mercaptan by DNA functionalized room temperature phosphorescent quantum dot nanocomposites

作者：Miao, Yanming^*; Wang, Ruirui; Yang, Wenli; Liu, Shuying; Yan, Guiqin

来源：Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy , 2020, 238: 118420.

DOI：10.1016/j.saa.2020.118420

• Single stranded DNA
• Room-temperature phosphorescent
• Ag
• Biological mercaptan

In this study, green low-toxicity Mn-doped Zns (Mn-Zns) room-temperature phosphorescent (RTP) quantum dots (QDs) (PQDs) nanocomposites (DNA-PQDs) were prepared under the optimal conditions by using single-stranded DNA (PS-C-ssDNA) rich of cytosine C and Thioguanine G (PS) as the template. DNA-PQDs interact with Ag+ to form Ag-N bonds and further produce C-Ag+-C conjugates. As a result, DNA-PQDs cluster together and induce the phosphorescent exciton energy transfer (PEET), resulting in quenching of room-temperature phosphorescent of DNA-PQDs. Nevertheless, Ag+ tends to form Ag-S bonds with biological mercaptan when it is added in, so that Ag+ falls from C-Ag+-C. DNA-PQDs changed from aggregation to looseness and RTP is recovered accordingly. On this basis, RTP detection of biologicalmercaptan is realized. Since this sensor systemhas RTP properties based on DNA-PQDs, it is very applicable to detection of mercaptan compounds in biological fluids.

• 单位
  山西师范大学