Effects of Huanglian Jiedu Decoction on the Metabolic Features of Brown Adipose Tissue Extracts in Fructose-induced Insulin Resistance Model Based on NMR Metabonomics

作者：YANG Yongxia; WANG Linlin; ZHENG Lingyun; WANG Shumei; HUANG Rongbo; ZHANG Lei; HUANG Yaoting

来源：高等学校化学学报, 2014, (9).

DOI：10.7503/cjcu20140308

• 1 H NMR
• Metabonomics
• Huanglian Jiedu decoction
• Insulin resistance

With the application of 1H nuclear magnetic resonance(1 H NMR) spectroscopy, we studied the effect of Huanglian Jiedu decoction( HJD) on the metabonomics of brown adipose tissue extracts in high fruc-tose-induced insulin resistance model. 32 Wistar rats were divided into four groups, i. e. , controls, model group, positive drug group and HJD treated group, 8 in each group. The last three groups were given 100 g/L fructose water for 28 d to establish insulin resistance model, normal control group was given the equal volume of pure water at the same time. After 28 d, four groups of rats were given 100 g/L fructose water continuously. Rats in positive drug group were administered orally atorvastain at a dose of 10 mg/( kg·d) , and rats in HJD treated group were given by gavage with HJD water. The control and model groups were given by gavage with a certain volume of saline solution, and the experiment lasted 56 d. Brown adipose tissues were obtained and 1 H NMR spectra of each sample was performed and analyzed by principal component analysis( PCA) method. Compared with the control group, lactate, alanine, choline, phosphocholine/glycerol phosphocholine ( PC/GPC) , creatine/creatinine, taurine and inosine increased in the model and lipid decreased. In comparison with model group, the myo-inositol was increased in HJD group, and HJD had reversed the metabolites in the model group. HJD can regulate the body’ s energy metabolism and reduce the damaged cell membrances and the injury of liver and kidney. Therefore, this study elucidates the metabolic mechanism of HJD on insulin re-sistance( IR) .

• 单位
  广东药学院