Human post-infection serological response to the spike and nucleocapsid proteins of SARS-CoV-2

作者：Xiao, Cheng; Ling, Shiman; Qiu, Minshan; Deng, Zhenxuan; Chen, Liping; Zhu, Airu; Chen, Yi; Liu, Yong; Lin, Xia; Lin, Fangmei; Wu, Qiubao; Shen, Lihan; Ye, Feng; Liu, Xiaoqing; Li, Yimin; Zhao, Jincun; Yang, Zifeng; Cowling, Benjamin J.; Webby, Richard; Zanin, Mark; Wong, Sook-San^*

来源：Influenza and Other Respiratory Viruses, 2021, 15(1): 7-12.

DOI：10.1111/irv.12798

• antibodies
• epidemiology
• nucleocapsid
• SARS-CoV-2
• serology
• spike

To inform seroepidemiological studies, we characterized the IgG- responses in COVID-19 patients against the two major SARS-CoV-2 viral proteins, spike (S) and nucleocapsid (N). We tested 70 COVID-19 sera collected up to 85 days post-symptom onset and 230 non-COVID-19 sera, including 27 SARS sera from 2003. Although the average SARS-CoV-2 S and N-IgG titers were comparable, N-responses were more variable among individuals. S- and N-assay specificity tested with non-COVID-19 sera were comparable at 97.5% and 97.0%, respectively. Therefore, S will make a better target due to its lower cross-reactive potential and its' more consistent frequency of detection compared to N.

• 单位
  广州医学院; 1; 东莞市人民医院