Cationic micelle-based siRNA delivery for efficient colon cancer gene therapy

作者：Lu, Yongping; Zhong, Lei; Jiang, Zhongliang; Pan, Haixia; Zhang, Yuanfa; Zhu, Guonian; Bai, Lan; Tong, Rongsheng; Shi, Jianyou^*; Duan, Xingmei^*

来源：Nanoscale Research Letters, 2019, 14: 193.

DOI：10.1186/s11671-019-2985-z

• Non-viral vector
• Gene therapy
• Gene delivery
• RNAi
• siRNA
• C26 colon cancer

Small interfering RNA (siRNA)-based gene therapy has provided an alternative strategy for cancer therapy. One of the key components within gene therapy process is the delivery system. As a novel non-viral gene vector, DMP, prepared by modifying mPEG-PCL micelle with cationic DOTAP lipid, has been prepared and successfully applied in plasmid DNA-based colon cancer gene therapy study. However, its potential in siRNA delivery is unknown. In this study, the preparation process of DMP was optimized and the anti-cancer efficacies of the DMP/siMcl1 and DMP/siBcl-xl complexes were studied on a mouse colon cancer model. Our results demonstrated that DMP cationic micelle-delivered siRNAs could effectively inhibit the growth of C26 colon cancer cells in vitro. Meanwhile, intratumoral administration of DMP/siMcl1 and DMP/siBcl-xl complexes obviously suppressed subcutaneous tumor model in vivo. These results suggest the DMP/siRNA complex to be a potential candidate for cancer gene therapy.

• 单位
  成都中医药大学; 四川省医学科学院（四川省人民医院）; 电子科技大学; 四川大学