Extraction and identification of anthocyanin from blueberries: effects of ultrasound-/microwave-assisted parameters

作者：Yang, Qi; Feng, ZhiQiang; Zhang, RuanBing; Wang, YunPu; Duan, DengLe^*; Wang, Qin; Zou, XiaoYu; Liu, YuHuan

来源：Pigment & Resin Technology, 2023.

DOI：10.1108/PRT-09-2022-0106

• Blueberry
• Anthocyanin
• Ultrasound-
• microwave-assisted extraction
• HPLC-QTOF-MS2

PurposeThis study aims to develop a green, economical and efficient ultrasonic-/microwave assisted extraction (UMAE) process for the extraction of anthocyanins. Design/methodology/approachAfter optimizing the extraction conditions by response surface methodology, three assays including DPPH, ABTS center dot+, FRAP were applied to analyze the antioxidant activity of the extracted anthocyanins. The stability under different temperatures, reductant concentrations and pHs was also discussed. The components of anthocyanins in blueberry were analyzed by HPLC-QTOF-MS2. FindingsThe optimal extraction parameters were ultrasonic power of 300 W, microwave power of 365.28 W and solid-liquid ratio of 30 (g/mL). The possible structures can be speculated as Delphinidin-3-O-galactoside, Delphinidin, Petunidin, Delphinidin-3-O-glucoside, Petunidin-3-O-glucoside, Cyanidin-3-O-glucoside. The results demonstrated that the UMAE can improve the yield of anthocyanins in shorter extraction time with higher activity. Originality/valueThe present study may provide a promising and feasible route for extracting anthocyanins from blueberries and studying their physicochemical properties, ultimately promoting the utilization of blueberry anthocyanins.

• 单位
  仲恺农业工程学院; 广东省农业科学院; 南昌大学