科研之友
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摘要
Modeling the assimilation of biodegradable contaminants by plants is a smart option to simulate the efficiency of phytoremediation in a field trial. The involvement of beta-cyanoalanine synthase (beta-CAS) and sulfurtransferase (ST) in cyanide -(CN-) assimilation in plants was evident. However, the contribution of both enzymes to -CN- assimilation by plants is not defined. In this study, a rice completely mixed reactor model (RCMRM) combined with boundary conditions is developed to mathematically examine the fraction of beta-CAS and ST involved in -CN- conversion in rice plants. The modeling steps include: 1) estimation of -CN- assimilation rates was based on the -CN- in plant biomass and the solution; 2) inputs of the mass balance equations of -CN- through either the beta-CAS pathway or the ST pathway were calculated individually; 3) the boundary conditions refer to the condition, in which the expression levels of beta-CAS and ST in the KCN treatments with application of exogenous 1-amino-cyclopropane-1-carboxylic acid (ACC) were higher than the KCN treatments without ACC addition. Results from the RCMRM suggested that -CN- assimilation rates by rice seedlings followed an exponential kinetic (first order), which was linearly increased in the first 2 h before more gradual until equilibrium was reached at about 12 h. The catalytic efficiency of beta-CAS activated in -CN- conversion was higher than that of ST, and the fraction of both enzymes to -CN- conversion is time-dependent rather than dose-dependent. Overall, this is the first attempt to mathematically describe the role of beta-CAS and ST to exogenous -CN- conversion in rice plants.
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单位桂林理工大学
