Optimization of flavonoid compound extraction from Ganoderma lucidum and its antioxidant activity on PC12 cells exposed to H2O2

作者：Wu, Linxiu; Liu, Xiaoyan^*; Wu, Siyi; Wang, Fan; Lu, Mengyao; Wang, Fanghua; Hu, Rongkang^*

来源：Journal of Food Measurement and Characterization, 2023.

DOI：10.1007/s11694-023-02163-5

• Antioxidant activity
• Extract
• Flavonoid
• Ganoderma lucidum
• Process optimization

Ganoderma lucidum has a high concentration of flavonoid compounds. The extraction procedure was optimized using a response surface methodology to achieve a high concentration of flavonoids from G. lucidum using ethanol as the extracting solvent, and the antioxidant activities were investigated. The highest flavonoids content was 0.56 +/- 0.03 mg/g when the solid-liquid ratio, extraction temperature, extraction time and ethanol concentration were 1:25 mg/L, 75 C-degrees and 1.5 h and 65%, respectively. The most influential factor was shown to be the solid-liquid ratio. FT-IR and H-1 NMR analysis of the purified ethanol extracts (PEE) revealed that it was primarily a 5, 7-Dihydroxyflavone. PEE exhibited significant DPPH center dot and hydroxyl radical scavenging activity, as well as stronger reducing capacity. Notably, in the presence of PEE, cell viability increased by 13.76% when compared to H2O2 treatment (p < 0.05), reaching 72.5%. In addition, when treated with the PEE, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH-Px) had significantly increased when compared to the H2O2-treated group (p < 0.01), which were 36.93, 15.01 and 15.11 U/g-protein. More importantly, PEE treatment could upregulate the expression of PI3K, Akt and downregulate the expression of Caspase-3 in PC12 cells stimulated with H2O2. As a result, PEE could be used as a novel source of natural antioxidants.