A rhodamine derivative-based fluorescent probe for visual monitoring of pH changes in the Golgi apparatus

作者：Feng, Zhongjiao; Wu, Jiasheng^*; Jiang, Meiyu; Sha, Jie; Liu, Weimin; Ren, Haohui; Zhang, Wenjun; Lee, Chun-Sing; Wang, Pengfei^*

来源：Sensors and Actuators, B: Chemical , 2022, 366: 131963.

DOI：10.1016/j.snb.2022.131963

• Fluorescent probe
• Rhodamine derivative
• Golgi apparatus
• pH probe

The pH homeostasis of Golgi plays a vital role in maintaining normal cell functions. An effective near-infrared fluorescent probe that can sense pH changes in Golgi apparatus is of great significance. Here, a Golgi-targeting fluorescent probe (Golgi-hNR) based on a rhodamine derivative is presented to show a highly sensi-tive response to pH variations. Golgi-hNR displays a conspicuous pH-dependent behavior at 664 nm from pH 7.4-3.0 with a remarkable fluorescence off-on response. The pKa value of Golgi-hNR is measured to be 3.62. Confocal fluorescence imaging reveals that Golgi-hNR diffuses into Golgi with high selectivity. Using Golgi-hNR, we have successfully visualized real-time tracking of Golgi pH variations for medical therapy in living cells.

• 单位
  中国科学院研究生院