Copper-containing chitosan-based hydrogels enabled 3D-printed scaffolds to accelerate bone repair and eliminate MRSA-related infection

作者：Zhang, Jinwei; Ye, Xiangling; Li, Wenhua; Lin, Zefeng; Wang, Wanshun; Chen, Lingling; Li, Qi; Xie, Xiaobo^*; Xu, Xuemeng^*; Lu, Yao^*

来源：International Journal of Biological Macromolecules, 2023, 240: 124463.

DOI：10.1016/j.ijbiomac.2023.124463

• Infected bone defect
• Methicillin-resistant Staphylococcus aureus
• (MRSA)
• 3D-printed scaffolds
• Hydrogels
• Copper

Bone defect combined with drug-resistant bacteria-related infection is a thorny challenge in clinic. Herein, 3Dprinted polyhydroxyalkanoates/8-tricalcium phosphate (PHA/8-TCP, PT) scaffolds were prepared by fused deposition modeling. Then copper-containing carboxymethyl chitosan/alginate (CA/Cu) hydrogels were integrated with the scaffolds via a facile and low-cost chemical crosslinking method. The resultant PT/CA/Cu scaffolds could promote not only proliferation but also osteogenic differentiation of preosteoblasts in vitro. Moreover, PT/CA/Cu scaffolds exhibited a strong antibacterial activity towards a broad-spectrum of bacteria including methicillin-resistant Staphylococcus aureus (MRSA) through inducing the intercellular generation of reactive oxygen species. In vivo experiments further demonstrated that PT/CA/Cu scaffolds significantly accelerated bone repair of cranial defects and efficiently eliminated MRSA-related infection, showing potential for application in infected bone defect therapy.

• 单位
  广州中医药大学; 南方医科大学; y; 5