Complex target SELEX-based identification of DNA aptamers against Bungarus caeruleus venom for the detection of envenomation using a paper-based device

作者：Anjali Anand; Bandhan Chatterjee; Abhijeet Dhiman; Renu Goel; Eshan Khan; Anita Malhotra; Vishal Santra; Nitin Salvi; M.V. Khadilkar; Ira Bhatnagar; Amit Kumar; Amit Asthana^*; Tarun Kumar Sharma^*

来源：Biosensors and Bioelectronics, 2021, 193: 113523.

DOI：10.1016/j.bios.2021.113523

• Aptamers
• SELEX
• Krait
• Snake venom
• Bungarotoxin
• Mass-spectrometry
• Paper device

Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is β-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.

• 单位
  2; Indian Institute of Technology; 1