An Investigation of Oxidative DNA Damage in Pharmacy Technicians Exposed to Antineoplastic Drugs in Two Chinese Hospitals Using The Urinary 8-OHdG Assay

HUANG Yu Wen; JIAN Le; ZHANG Mei Bian; ZHOU Quan; YAN Xiao Feng; HUA Xu Dong; ZHOU Ying; HE Ji Liang; The Second Affiliated Hospital of College of Medicine of Zhejiang University; Hangzhou; Zhejiang; China School of Public Health; Curtin Health Innovation Research Institute; Curtin University of Technology; Perth; Australia Zhejiang Provincial Center for Disease Control; Prevention; Hangzhou; Zhejiang

中国知网

摘要

Objective To investigate oxidative DNA damage in pharmacy technicians preparing antineoplastic drugs at the PIVAS (Pharmacy Intravenous Admixture Service) in two Chinese hospitals.Methods Urinary 8-OHdG served as a biomarker.5-Fluorouracil (5-FU) concentrations in air,masks and gloves were determined.The spill exposure of each PIVAS technician to antineoplastic drugs was investigated.Eighty subjects were divided into exposed group I,II,and control group I,II.Results 5-FU concentration ratios for gloves and masks in exposed group I were significantly higher than those in exposed group II (P<0.05 or P<0.01).The average urinary 8-OHdG concentrations in exposed group I,control group I,exposed group II,and control group II were 14.69±0.93,10.68±1.07,10.57±0.55,and 11.96±0.73 ng/mg Cr,respectively.Urinary 8-OHdG concentration in exposed group I was significantly higher than that in control group I or that in exposed group II (P<0.01).There was a significant correlation between urinary 8-OHdG concentrations and spill frequencies per technician (P<0.01).Conclusion There was detectable oxidative DNA damage in PIVAS technicians exposed to antineoplastic drugs.This oxidative DNA damage may be associated with their spill exposure experience and contamination of their personal protective equipment.

关键词

Urinary 8-OHdG Oxidative DNA damage Antineoplastic drugs Occupational exposure Pharmacy Intravenous Admixture Service