Improvement in lipase-catalyzed methanolysis of triacylglycerols for biodiesel production using a solvent engineering method

作者：Su Erzheng; Wei Dongzhi^*

来源：Journal of Molecular Catalysis - B Enzymatic, 2008, 55(3-4): 118-125.

DOI：10.1016/j.molcatb.2008.03.001

• Solvent engineering
• Co-solvent mixture
• Biodiesel
• Novozym435
• Jatropha curcas L. seed oil

A solvent engineering strategy was applied to the lipase-catalyzed methanolysis of triacyl glycerols for biodiesel production. The effect of different pure organic solvents and co-solvent mixtures on the methanolysis was compared. The substrate conversions in the co-solvent mixtures were all higher than those of the corresponding pure Organic solvents. Further study showed that addition of co-solvent decreased the values of vertical bar log P-interface - log P-substrate vertical bar and thus led to a faster reaction. The more the values of vertical bar log(interface) - logP(substrate)vertical bar decreased, the faster the reaction proceeded and the higher the conversion attained. Different co-solvent ratio was further investigated. The co-solvent mixture of 25% t-pentanol:75% isooctane (v/v) was optimal. with which both the negative effects caused by excessive methanol and by-product glycerol could be eliminated. There was no obvious loss in lipase activity even after being repeatedly used for 60 cycles (720 h) with this co-solvent mixture as reaction medium. Other lipases and lipase combinations can also catalyze methanolysis in this co-solvent mixture. Furthermore, other vegetable oils were also explored for biodiesel production in this co-solvent Mixture and it had been found that this co-solvent mixture media has extensive applicability.

• 单位
  东华大学