Influence of DNA methyltransferase 3b on FHIT expression and DNA methylation of the FHIT promoter region in hepatoma SMMC-7721 cells

作者：Jia Xiang Wang; Yong Gan Zhang; Long Shuan Zhao Department of Surgery; First Affiliated Hospital; Zhengzhou University; Zhengzhou; China

来源：HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL, 2009, (03): 273-277.

• liver neoplasms
• DNA methyltransferase 3b
• fragile histidine trial
• methylation
• gene expression

BACKGROUND:Alterations in DNA methylation occur during the pathogenesis of human tumors.In this study, we investigated the influence of DNA methyltransferase 3b(DNMT3b)on fragile histidine trial(FHIT)expression and on DNA methylation of the FHIT promoter region in the hepatoma cell line SMMC-7721. METHODS:DNMT3b siRNA was used to down-regulate DNMT3b expression.DNMT3b and FHIT proteins were determined by Western blotting.Methylation-specific PCR was used to analyze the methylation status of the FHIT gene. R...