Research on the mechanism of fluorescence quenching of HRP by NaN3

Authors：Xia Bing Le; Peng Dun Geng; Niu Hai Xia; Liu Qing Liang

Source：Spectroscopy and Spectral Analysis, 2005, 25(3).

The fluorescence and synchronous fluorescence spectra were employed to study the binding between sodium azide (NaN3) and horseradish peroxidase (HRP), which was affected by the molecular conformation and the microenvironment of the fluorescence residues. The mechanism of the fluorescence quenching of HRP by NaN3 was discussed and the binding constant and the number of binding sites of non-covalent bond between them were calculated.