Enhancement and modulation of enzymatic activity through higher-order structural changes of giant DNA-protein multibranch conjugates

摘要

A giant DNA leap for enzyme-kind: Giant DNA-protein multibranch conjugates were shown to be composed of a single β-lactamase enzyme (green, see scheme) conjugated to up to four 48.5 kbp lambda phage DNA branches (blue lines). The conjugation of giant DNA induces a two- to fourfold enhancement of the enzymatic activity, which is modulated by changes in the higher-order DNA structure.

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