Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons

作者：Sun, Zhao-Wei; Waybright, Jarod M.; Beldar, Serap; Chen, Lu; Foley, Caroline A.; Norris-Drouin, Jacqueline L.; Lyu, Tian-Jie; Dong, Aiping; Min, Jinrong; Wang, Yu-Pu; James, Lindsey, I^*; Wang, Yun

来源：Advanced Science, 2022, 9(10): 2104317.

DOI：10.1002/advs.202104317

• chromodomain Y-like
• epigenetic regulation
• K(v)2
• 1 channel
• novel chromodomain Y-like antagonist
• pain sensation

Epigenetic modifications are involved in the onset, development, and maintenance of pain; however, the precise epigenetic mechanism underlying pain regulation remains elusive. Here it is reported that the epigenetic factor chromodomain Y-like (CDYL) is crucial for pain processing. Selective knockout of CDYL in sensory neurons results in decreased neuronal excitability and nociception. Moreover, CDYL facilitates histone 3 lysine 27 trimethylation (H3K27me3) deposition at the Kcnb1 intron region thus silencing voltage-gated potassium channel (K-v) subfamily member K(v)2.1 transcription. Loss function of CDYL enhances total K-v and K(v)2.1 current density in dorsal root ganglia and knockdown of K(v)2.1 reverses the pain-related phenotypes of Cdyl deficiency mice. Furthermore, focal administration of a novel potent CDYL antagonist blunts nociception and attenuates neuropathic pain. These findings reveal that CDYL is a critical regulator of pain sensation and shed light on the development of novel analgesics targeting epigenetic mechanisms.

• 单位
  y; 5; 1; 北京大学