Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA

作者：Yan Qin Lu; School of Medicine; Shandong University; Jinan; China; Shandong Medicinal Biotechnology Center; Shandong Academy of Medical Science; Key laboratory of Ministry of Health for Biotech Drug; Jinan; Shandong Province; China Jin Xiang Han; Peng Qi; Bo Zhu; Shandong Medicinal Biotechnology Center; Shandong Academy of Medical Sciences; Key Laboratory of Ministry of Health for Biotech Drugs; Jinan; Shandong Province; China Wei Xu; Yan Hui Zu

来源：World Journal of Gastroenterology, 2006, (45): 7365-7370.

• Hepatitis B virus
• Serum DNA
• Real-time PCR
• Extraction method

AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum samples derived from the three different standard curves using certain serum DNA. Then the efficiency of six HBV DNA...