MiRNA-338-3p Inhibits Neuroinflammation in the Corpus Callosum of LCV-LPS Rats Via STAT1 Signal Pathway

作者：Liu, Nan; Zhou, Qiuping; Wang, Huifang; Li, Qian; Chen, Zhuo; Lin, Yiyan; Yi, Lingling; Jiang, Shuqi; Chen, Chunbo^*; Deng, Yiyu^*

来源：Cellular and Molecular Neurobiology, 2023, 43(7): 3669-3692.

DOI：10.1007/s10571-023-01378-w

• MiR-338-3p
• STAT1
• Neuroinflammation
• Astrocytes
• BV2 cells

Neuroinflammation is a common characteristic of intracranial infection (ICI), which is associated with the activation of astrocytes and microglia. MiRNAs are involved in the process of neuroinflammation. This study aimed to investigate the potential mechanism by which miR-338-3p negatively modulate the occurrence of neuroinflammation. We here reported that the decreased levels of miR-338-3p were detected using qRT-PCR and the upregulated expression of TNF-alpha and IL-1 beta was measured by ELISA in the cerebrospinal fluid (CSF) in patients with ICI. A negative association between miR-338-3p and TNF-alpha or IL-1 beta was revealed by Pearson correlation analysis. Sprague-Dawley (SD) rats were injected with LPS (50 mu g) into left cerebral ventricule (LCV), following which the increased expression of TNF-alpha and IL-1 beta and the reduction of miR-338-3p expression were observed in the corpus callosum (CC). Moreover, the expression of TNF-alpha and IL-1 beta in the astrocytes and microglia in the CC of LCV-LPS rats were saliently inhibited by the overexpression of miR-338-3p. In vitro, cultured astrocytes and BV2 cells transfected with mimic-miR-338-3p produced less TNF-alpha and IL-1 beta after LPS administration. Direct interaction between miR-338-3p and STAT1 mRNA was validated by biological information analysis and dual luciferase assay. Furthermore, STAT1 pathway was found to be implicated in inhibition of neuroinflammation induced by mimic miR-338-3p in the astrocytes and BV2 cells. Taken together, our results suggest that miR-338-3p suppress the generation of proinflammatory mediators in astrocyte and BV2 cells induced by LPS exposure through the STAT1 signal pathway. MiR-338-3p could act as a potential therapeutic strategy to reduce the neuroinflammatory response.

• 单位
  南方医科大学; 广东省人民医院