Rapid Detection of the Monkeypox Virus Genome and Antigen Proteins Based on Surface-Enhanced Raman Spectroscopy

作者：Zhang, Zhe; Jiang, Heng; Jiang, Shen; Dong, Tuo; Wang, Xiaotong; Wang, Yunpeng; Li, Yang^*

来源：ACS applied materials & interfaces, 2023, 15(29): 34419-34426.

DOI：10.1021/acsami.3c04285

• surface-enhanced Raman spectroscopy
• monkeypox virus
• virus detection
• biosensor
• machine learning
• label-free detection

The conventional detection methods cannot satisfy theneed forearly and rapid detection of monkeypox virus (MPXV) infection. Thisis due to complicated pretreatment, time consumption, and complexoperation of the diagnostic tests. Based on surface-enhanced Ramanspectroscopy (SERS), this study attempted to capture the characteristicfingerprints of the MPXV genome and multiple antigenic proteins withoutthe need to design specific probes. The minimum detection limit ofthis method is 100 copies/mL, with good reproducibility and signal-to-noiseratio. Therefore, the relationship between characteristic peak intensityand the protein and nucleic acid concentration can be used to constructa concentration-dependent spectral line with a good linear relationship.Additionally, principal component analysis (PCA) could identify theSERS spectra of four different MPXV proteins in serum. Therefore,this rapid detection method in the current outbreak of monkeypox controland the future response to possible new outbreaks has broad applicationprospects.

• 单位
  哈尔滨医科大学; y