Structural characterization and anti-photoaging activity of a polysaccharide from Sargassum fusiforme

摘要

In this study, a purified algal polysaccharide (P1) was isolated from Sargassum fusiforme and its structural characteristics and anti-photoaging activity were studied. Results showed that P1 had a molecular weight of 289 kDa and was mainly composed of mannuronic acid, guluronic acid and fucose with molar ratio of 7.67:2.35:1.00. The backbone of P1 was -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 -> 4)-beta-ManA-(1 -> 4)-beta-ManA-(1 -> 4)-alpha-GulA-(1 ->)-beta-ManA-(1 -> 3,4)-beta-ManA-(1 -> with a terminal group of alpha-Fucp-(1 -> linked to O-3 position of -> 3,4)-beta-ManA-(1 ->. In addition, P1 could inhibit the expressions of MMPs (MMP-1, MMP-3 and MMP-9) in the UVB-irradiated HaCaT cells, indicating that P1 could reduce collagen loss caused by UVB irradiation. It also reduced the contents of ROS and inflammatory factors (TNF-alpha, IL-6 and IL-1 beta), indicating that P1 could reduce the oxidative stress and inflammation response. Thus, Sargassum fusiforme polysaccharide P1 could be used as a potential functional food to relieve skin photoaging.

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