Carbon Nanospheres Exert Antitumor Effects Associated with Downregulation of 4E-BPI Expression on Prostate Cancer

Authors：Dong, Weimin; Luo, Yong; Zhang, Guian; Zhang, Hui; Liang, Yuxiang; Zhuo, Yangjia; Liang, Yingke; Zou, Fen^*; Zhong, Weide^*

Source：International Journal of Nanomedicine, 2020, 15: 5545-5559.

DOI：10.2147/IJN.S257522

• carbon nanosphere
• prostate cancer
• antitumor
• 4E-BPI

Introduction: Although carbon nanospheres (CNPs) are promising nanomaterials in cancer treatment, how they affect prostate cancer (PCa) remains unclear. @@@ Methods: In this study, scanning electron microscopy (SEM), X-ray diffraction (XRD), and Raman spectroscopy were used to confirm the successful synthesis of CNPs. CCK-8, flow cytometry, Transwell, wound healing, Western blot and immunohistochemistry (IHC) assays were performed to evaluate the antitumor effect of CNPs toward the two kinds of prostate cancer cell lines PC3 and DU145. @@@ Results: Our results showed that CNPs inhibited cell growth, invasion, and migration and induced apoptosis and autophagy in PCa cells. Multifactor detection of a single Akt phosphorylation pathway and Western blot results suggested the suppression of 4E-BPI in PCa cells after incubation with CNPs. The results from animal experiments also suggested the antitumor effect of CNPs and reduced 4E-BPI expression in PCa tissue samples from BALE/c nude mice administered a local subcutaneous injection of CNPs.

• Institution
  广州医学院; 1; 南方医科大学