The poly-thymine based DNA photolithography onto electrostatic coupling substrates

作者：Youngjun Song

来源：MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2020, 111: 110795.

DOI：10.1016/j.msec.2020.110795

• DNA
• Self-assembly
• Patterning
• Thymine dimerization
• Hybridization chain reaction

In order to develop a rapid and high fidelity process for DNA self-assembly with patterning, the pattern of thymine dimerization is presented onto electrostatically bound DNA substrate by photolithography. The ability of binding for the process, which is attenuated conditions such as contact with photomask and washing by solution buffer is evaluated by X-ray photoelectron spectroscopy (XPS). Through thymine dimerization and hybridization chain reaction (HCR), DNA patterns, including multi-patterns, are demonstrated. For expansion to protein molecular patterning, the target DNA is tethered to biotin, allowing patterning with streptavidin linked fluorophores such as Cy3-streptavidin and phyecocayine-streptavidin.