KLF16 inhibits PEDV replication by activating the type I IFN signaling pathway

作者：Dong, Sujie; Kong, Ning; Shen, Haiyan; Li, Youwen; Qin, Wenzhen; Zhai, Huanjie; Zhai, Xueying; Yang, Xinyu; Ye, Chenqian; Ye, Manqing; Liu, Changlong; Yu, Lingxue; Zheng, Hao; Tong, Wu; Yu, Hai; Zhang, Wen^*; Tong, Guangzhi^*; Shan, Tongling^*

来源：Veterinary Microbiology, 2022, 274: 109577.

DOI：10.1016/j.vetmic.2022.109577

• KLF16
• PEDV
• TRAF6
• IFN
• Replication

KLF16, a member of KLFs (Kruppel-like factors), contributes to the progression of a variety of cancer types. There is, however, still uncertain regarding the role of KLF16 in viral replication and the signaling mechanism of type I IFN. It was discovered that KLF16 inhibited the replication of porcine epidemic diarrhea virus (PEDV) through the type I IFN signaling pathway. Besides, it can also be found that the expression of KLF16 was down-regulated after PEDV infection of LLC-PK1 cells. Furthermore, overexpression of KLF16 inhibited the replication of PEDV in Vero cells as well as LLC-PK1 cells, whereas the replication of PEDV was promoted by the knockdown of KLF16. KLF16 up-regulated the expression of interferons (IFNs) via the TRAF6-pTBK1-pIRF3 pathway with the aim of promoting the host antiviral innate immune response. In addition, the obtained findings proved that KLF16 plays a novel role in antiviral action, thereby offering novel possibilities for preventing and controlling PEDV.

• 单位
  江苏大学; 广东省农业科学院