The cardioprotective effects of cerium oxide nanoparticles against the poisoning generated by aluminum phosphide pesticide: Controlling oxidative stress and mitochondrial damage

作者：Yang, Yongzheng; Bustani, Ghadeer Sabah; Alawsi, Taif; Altalbawy, Farag M. A.; Kareem, Ali Kamil; Gupta, Jitendra; Zhu, Ping^*; Hjazi, Ahmed; Alawadi, Ahmed Hussien; Mustafa, Yasser Fakri

来源：Pesticide Biochemistry and Physiology, 2023, 197: 105701.

DOI：10.1016/j.pestbp.2023.105701

• Aluminum phosphide
• Apoptosis
• CeO 2 nanoparticle
• Mitochondrial markers
• Oxidative stress

Background: Aluminum phosphide (AlP) is a well-known toxic compound used as an agricultural pesticide to prevent insect damage to stored crops. However, even if just a small amount was consumed, it caused lasting harm to the human body and, in acute concentrations, death. The current study employed cerium oxide nano -particles (CeO2 NPs) to reduce oxidative stress and various harmful outcomes of AlP poisoning.Methods: Following finding effective concentrations of CeO2 NPs via MTT assay, Human Cardiac Myocyte (HCM) cells were pre-treated with CeO2 NPs for 24 h. After that, they were exposed to 2.36 mu M AlP. The activity of oxidative stress and mitochondrial biomarkers, including mitochondrial swelling, mitochondrial membrane potential, and cytochrome c release, were evaluated in HCM cells. Finally, the population of apoptotic and necrotic cells was assessed via flow cytometry.Results: After 24 h, data revealed that all tested concentrations of CeO2 NPs were safe, and 25 and 50 mu M of that were selected as effective concentrations. Oxidative stress markers (malondialdehyde, protein carbonyl, super-oxide dismutase, and catalase) showed that CeO2 NPs could successfully decrease AlP poisoning due to their antioxidant characteristics. Mitochondrial markers were also recovered by pre-treatment of HCM cells with CeO2 NPs. Furthermore, pre-treating with CeO2 NPs could compensate for the reduction of live cells with AlP and cause a diminishing in the population of early and late apoptotic cells. Conclusion: As a result, it is evident that CeO2 NPs, through the recovery of oxidative stress and mitochondrial damages caused by AlP, reduce apoptosis and have therapeutic potentials on HCM cells.

• 单位
  南方医科大学; 广东省心血管病研究所; 广东省人民医院