Anti-fatigue and Anti-aging Effects of Coreopsis tinctoria Flavonoids in Mice

摘要

The objectives of this study were to observe the effects of the Coreopsis tinctoria flavonoids (CTFs) on anti-fatigue in common mice and anti-aging in the aging model mice. The common mice were randomly divided into CTFs-treated groups of low (100mg/kg.d), medium (200 mg/kg.d), and high (400 mg/ kg.d) dose and the control group, all the mice were intragastric administration once daily for 21 days. The anti-fatigue effects of the CTFs were designed by using the weight-bearing swimming experiment in mice and then the determination of the serum urea nitrogen (SUN), blood lactic acid (BLA), hepatic glycogen (HGn) and muscle glycogen (MGn) in mice after exercise. The anti-aging effect of the CTFs was investigated by the aging model mice induced by D-galactose. The study was carried out to investigate the immune organ indexes, cerebral index (CI), the contents of malondialdehyde (MDA) in the serums, hydroxy radical (center dot OH) in the liver tissues, hydrogen peroxide (H2O2) in the brain tissues, and the activities of superoxide dismutase (SOD) and catalase (CAT) in the liver tissues of the aging mice administered with D-galactose in vivo by subcutaneous injection together with the CTFs at the three doses mentioned before by intragastric administration once daily for 6 weeks. The anti-fatigue results showed that the CTFs could considerably prolong the weight-bearing swimming time, decrease the contents of the BLA and SUN, and increase the contents of the MGn and HGn after exercise compared with those of the control group. And then these results suggested that the CTFs can enhance the anti-fatigue ability of mice. The anti-aging results showed that the three doses of the CTFs could all decrease the contents of H2O2 in the brain tissues, center dot OH in the liver tissues and MDA in the serums, increase the CI, spleen index (SI), thymus index (TI) and the activities of SOD and CAT in the liver tissues very significant (p < 0.01) compared with those of the aging mice. The above results suggested that the CTFs might have anti-aging effects by improving the human body's antioxidant capacity.

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