Nitrogen and boron co-doped graphene nanoribbons as peroxidase-mimicking nanozymes for enhanced biosensing

作者：Luo, Siyu; Sha, Meng; Tian, Fei; Li, Xialian; Fu, Lijie; Gu, Yingqiu; Qu, Lu-Lu^*; Yang, Guo-Hai^*; Zhu, Chengzhou^*

来源：CHINESE CHEMICAL LETTERS, 2022, 33(1): 344-348.

DOI：10.1016/j.cclet.2021.06.018

• Graphene nanoribbons
• Heteroatom doping
• Nanozymes
• Biosensing
• Interleukin-6

The rational design of nanozymes with superior activities is essential for improving bioassay performances. Herein, nitrogen and boron co-doped graphene nanoribbons (NB-GNRs) are prepared by a hydrothermal method using urea as the nitrogen source and boric acid as the boron source, respectively. The introduction of co-doped and edge structures provides high defects and active sites. The resultant NB-GNRs nanozymes show superior peroxidase-like activities to nitrogen-doped and boron-doped counterparts due to the synergistic effects. By taking advantage of their peroxidase-like activities, NB-GNRs are used for the first time to develop enzyme-linked immunosorbent assay for the detection of interleukin-6. The biosensors exhibit a high performance with a linear range from 0.001 ng/mL to 1000 ng/mL and a detection limit of 0.3 pg/mL. Due to their low cost and high stability, the proposed nanomaterials show great promise in biocatalysis, immunoassay development and environmental monitoring.