"French fries"-like luminescent metal organic frameworks for the fluorescence determination of cytochrome c released by apoptotic cells and screening of anticancer drug activity

作者：Xie, Ruirui; Liu, Yanyan; Yang, Peipei; Huang, Lan; Zou, Xun; Liu, Jiamin; Ren, Qingfan; Tao, Jia^*; Zhao, Peng^*

来源：Mikrochimica Acta, 2020, 187(4): 221.

DOI：10.1007/s00604-020-4207-x

• Cytochrome c
• Metal organic framework
• Quantum dots
• Apoptosis
• Anticancer drug
• Fluorescence

A luminescent metal organic framework was prepared by encapsulating Zn-Ag-In-S quantum dots into "French fries"-like MIL-68(In) metal organic frameworks (ZAISQDs@MIL-68(In)). The ZAISQDs@MIL-68(In) had a maximum excitation wavelength at 370 nm and maximum emission wavelength at 620 nm. It was found that the ZAISQDs@MIL-68(In) was efficiently quenched by cytochrome c (Cyt c), which is an important biomarker of early cell apoptosis. The quenching mechanism was ascribed to be an inner filter effect and dynamic quenching of Cyt c towards the ZAISQDs@MIL-68(In), and the enrichment effect of MIL-68(In). Benefiting from the multiple advantages, ZAISQDs@MIL-68(In) was developed as an assay strategy of Cyt c with logarithmic relation between signal quenching and concentration in the range 0.02 to 3.5 mu M. The linear equation was (F-0-F)/F-0 = 0.5043 + 0.2678 x logc(Cyt c) with a detection limit of 8 nM. Cyt c released by drug induced apoptotic cells was determined by ZAISQDs@MIL-68(In), and this strategy has been utilized for the screening of anticancer drug activity.

• 单位
  南方医科大学