Characterization of DNA Intercalator Bound to Calf Thymus DNA:Ionic Strength and pH Value Effects

摘要

By means of circular dichroism(CD) spectrum coupled with UV-Vis and fluorescence spectra,the binding model of DNA intercalator A1{4-(2-diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile} to calf thymus(CT) DNA was investigated,depending on the values of R(R is defined as the ratio of the concentration of A1 to CT DNA base pairs) and different outer factors.Molecules A1 were intercalated into the CT DNA base pairs in different orientations in the intercalation pocket at a lower R value...

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