Characterization of DNA Intercalator Bound to Calf Thymus DNA:Ionic Strength and pH Value Effects

作者：YUAN Chun li; ZHANG Zhi chao; SONG Ting; WU Gui ye Department of Gynecology; Obstetrics; First Hospital of Jilin University; Changchun; P R China; State Key Laboratory of Fine Chemicals; Dalian University of Technology; Dalian; P R China

来源：Chemical Research in Chinese Universities, 2009, (04): 492-496.

• DNA intercalator
• 4-(2-Diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1
• 2-b]pyrrole-9-carbonitrile
• Io-nic strength

By means of circular dichroism(CD) spectrum coupled with UV-Vis and fluorescence spectra,the binding model of DNA intercalator A1{4-(2-diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile} to calf thymus(CT) DNA was investigated,depending on the values of R(R is defined as the ratio of the concentration of A1 to CT DNA base pairs) and different outer factors.Molecules A1 were intercalated into the CT DNA base pairs in different orientations in the intercalation pocket at a lower R value...