A dual responsive fluorescent probe for selective detection of cysteine and bisulfite and its application in bioimaging

Authors：Wang, Xiaofeng; Li, Mingshun; Duan, Tingting^*; Zou, Yuxia^*; Zhou, Xuejun^*

Source：2021, 12(2): 874-877.

DOI：10.1039/d1ra08317a

• SULFUR-DIOXIDE
• OXIDATIVE DAMAGE
• HOMOCYSTEINE

A coumarin-based dual responsive fluorescent probe with a simple structure was developed for the detection of Cys and HSO3-. Under simulated physiological conditions, Cou-F displayed an on-off fluorescence response to Cys at 521 nm and an off-on fluorescence response to HSO3- at 500 nm. Furthermore, Cou-F had the advantages of high sensitivity, strong specificity and rapid response. The detection limits of Cou-F toward Cys and HSO3- were 0.54 mu M and 0.65 mu M, respectively. Cou-F enabled high selective responses to Cys and HSO3- over other biologically related species. The response times of Cou-F toward Cys and HSO3- were 80 s and 100 s. The fluorescence imaging of Cys and HSO3- was achieved in living RAW246.7 cells.

• Institution
  海南医学院; 南方医科大学; 1