Accurate targeted long-read DNA methylation and hydroxymethylation sequencing with TAPS.

作者：Liu, Yibin; Cheng, Jingfei; Siejka-Zielinska, Paulina; Weldon, Carika; Roberts, Hannah; Lopopolo, Maria; Magri, Andrea; D'Arienzo, Valentina; Harris, James M; McKeating, Jane A; Song, Chun-Xiao^*

来源：Genome Biol, 2020, 21(1): 54.

DOI：10.1186/s13059-020-01969-6

• 5-Methylcytosine
• Bisulfite-free
• DNA methylation
• Epigenetic phasing
• Long-read sequencing

We present long-read Tet-assisted pyridine borane sequencing (lrTAPS) for targeted base-resolution sequencing of DNA methylation and hydroxymethylation in regions up to 10 kb from nanogram-level input. Compatible with both Oxford Nanopore and PacBio Single-Molecule Real-Time (SMRT) sequencing, lrTAPS detects methylation with accuracy comparable to short-read Illumina sequencing but with long-range epigenetic phasing. We applied lrTAPS to sequence difficult-to-map regions in mouse embryonic stem cells and to identify distinct methylation events in the integrated hepatitis B virus genome.