Microwave-Assisted Synthesis of Arene Ruthenium(II) Complex as Apoptosis Inducer of A549 Cells

作者：Wu Qiong; Wu Jian; Mei Wen Jie^*; Wang Qi; Zhang Zhao; Wu Xiao Hui; Sun Fen Yong; Wu Wei Li; Chen Yan Hua; Hu Xiao Ying; Tao Yun Yi

来源：Australian Journal of Chemistry: an international journal for chemical science , 2013, 66(11): 1422-1427.

DOI：10.1071/CH13269

An arene ruthenium(ii) complex coordinated with 2-(2-chlorophenyl)-1H-imidazo[4,5-f][1,10]phenanthroline, [((6)-C6H6)Ru(o-ClPIP)Cl]Cl (1), has been prepared by using microwave-assisted synthesis technology. The anti-tumour activity of this complex against various tumour cells has been evaluated by MTT assay and the results show that complex 1 exhibits selective inhibitory activity against the growth of human lung adenocarcinoma A549 cells with IC50=31.58M. Further studies by flow cytometric analysis showed that apoptosis of A549 cells was observed when dealt with complex 1. Furthermore, complex 1 exhibits excellent binding affinity with DNA molecules which was confirmed by spectroscopy methods, as well viscosity and melting point experiments. As a result, the conformation of DNA molecules was disturbed by complex 1.

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  广东药学院