New universal matK primers for DNA barcoding angiosperms

摘要

The chloroplast maturase K gene(matK) is one of the most variable coding genes of angiosperms and has been suggested to be a barcode for land plants.However,matK exhibits low amplification and sequencing rates due to low universality of currently available primers and mononucleotide repeats.To resolve these technical problems,we evaluated the entire matK region to find a region of 600-800 bp that is highly variable,represents the best of all matK regions with priming sites conservative enough to design univ...

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