Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition

作者：Chen, Jiali; Wang, Yifan; Lin, Shuru; Yu, Quanxiang; Qi, Zhengfei; Jiang, Wenqi; Zhao, Qiang^*; Fu, Qingshan Bill^*

来源：Biochemical and Biophysical Research Communications, 2024, 694: 149395.

DOI：10.1016/j.bbrc.2023.149395

• SpoVM
• SpoIVA
• Purification
• Bicelles
• Interaction
• SpoVMP9A

SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVMP9A were obtained by cyanogen bromide cleavage and reconstituted into bicelles. The purification of SpoIVA was achieved through a rigorous process involving NiNTA chromatography column and size exclusion chromatography. This study utilized Biacore to obtain a direct determination of the kinetic parameters of interaction between SpoVM (SpoVMP9A) and SpoIVA in Bicelle conditions.

• 单位
  南方医科大学; 中国科学院