Characteristics of cold plasma treatment and enzymatic hydrolysis on IgG/ IgE-binding ability of ?-lactoglobulin

作者：Liu, Zhi-Wei^*; Zhang, Lin -Lin; Zhou, Ying-Xue; Tang, Pan-Pan; Tan, Yi-Cheng; Cheng, Jun-Hu; Khaneghah, Amin Mousavi; Aadil, Rana Muhammad^*

来源：Food Bioscience, 2022, 50: 102161.

DOI：10.1016/j.fbio.2022.102161

• ?-lactoglobulin
• Cold plasma
• Enzyme hydrolysis
• Antigenicity

Characteristics of cold plasma (CP) treatment and enzymatic (papain and alcalase) hydrolysis on IgG/IgE-binding ability beta-lactoglobulin (beta-LG) were compared, which is analyzed by immunoblot, WB, ELISA, and LC-MS/MS analysis. Results indicated that CP treatment exhibit similar characteristic to enzymatic hydrolysis to cleave the polypeptides chain of beta-LG for reduction of its IgG/IgE binding ability. A similar tendency of decline in IgG/ IgE binding capacity of beta-LG after CP treatment was achieved in comparison with alcalase hydrolysis, which was reduced by 57.69% (IgG) and 56% (IgE) (for 4 min CP treatment) and 61.89% (IgG) and 27.54% (IgE) for alcalase hydrolysis (75 min), while no significant difference was detected for papain hydrolysis. Immunoblot and LC-MS/MS analysis confirmed that the antigenicity reduction was ascribed to the breakdown of the IgG/IgE linear epitopes of beta-LG. Further combined with CP treatment (1 min) with alcalase hydrolysis (75 min), the reduction of antigenicity of beta-LG reached 68.46% (IgG) and 59.26% (IgE). This study suggests that CP treatment is a promising alternative to enzymatic hydrolysis for hypoallergenic protein-producing.

• 单位
  湖南农业大学