ATP-Triggered Intracellular In Situ Aggregation of a Gold-Nanoparticle-Equipped Triple-Helix Molecular Switch for Fluorescence Imaging and Photothermal Tumor Therapy

作者：Jiang, Yao; Zhao, Wenjing; Zhou, Huimin; Zhang, Qiuqi; Zhang, Shusheng^*

来源：Langmuir : the ACS journal of surfaces and colloids , 2022, 38(12): 3755-3764.

DOI：10.1021/acs.langmuir.1c03331

• DNA
• RELEASE
• PHOTOSENSITIZER
• NANOPLATFORM

Isotropic gold nanoparticles (AuNPs) can generate a plasma-plasma interaction when aggregating and can also produce ideal photothermal effects. Some studies have designed ATP-responsive nanodrug delivery systems by taking advantage of the differences between internal and external ATP in tumor cells, but few studies have focused on the photothermal effects of ATP-induced AuNP aggregation in tumors. Here, a triple-helix probe (THP) molecular switch and MUC1 aptamer-functionalized AuNPs were constructed for fluorescence imaging analysis and photothermal therapy (PTT). The MUC1 aptamer guides THP-AuNP targeting in tumor cells, followed by the high concentration of ATP inducing structural changes in triple-helix probes and causing the intracellular aggregation of AuNPs, which cannot escape from the tumor site, enabling tumor imaging while performing PTT. Therefore, the designed THP-AuNPs have promising applications in fluorescence imaging and PTT.

• 单位
  南方医科大学; 1; 山东大学