Strong Protein Adhesives through Lanthanide-enhanced Structure Folding and Stack Density

摘要

Generating strong adhesion by engineered proteins has the potential for high technical applications. Current studies of adhesive proteins are primarily limited to marine organisms, e.g., mussel adhesive proteins. Here, we present a modular engineering strategy to generate a type of exotic protein adhesives with super strong adhesion behaviors. In the protein complexes, the lanmodulin (LanM) underwent & alpha;-helical conformational transition induced by lanthanides, thereby enhancing the stacking density and molecular interactions of adhesive protein. The resulting adhesives exhibited outstanding lap-shear strength of & AP;31.7 MPa, surpassing many supramolecular and polymer adhesives. The extreme temperature (-196 to 200 & DEG;C) resistance capacity and underwater adhesion performance can significantly broaden their practical application scenarios. Ex vivo and in vivo experiments further demonstrated the persistent adhesion performance for surgical sealing and healing applications. @@@ A modular engineering strategy was developed to fabricate exotic protein adhesives with ultra-strong adhesion performance, extreme-temperature resistance, and underwater stability. The lanthanide-induced conformational transition enhanced the stacking density of adhesive protein moieties, thus endowing stronger molecular interactions. This work offers a versatile approach to fabricate robust bio-adhesives with multiple desired properties.+image

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