Irx5 and transient outward K+ currents contribute to transmural contractile heterogeneities in the mouse ventricle

作者：Kim, Kyoung-Han^*; Oh, Yena; Liu, Jie; Dababneh, Saif; Xia, Ying; Kim, Ri Youn; Kim, Dae-Kyum; Ban, Kiwon; Husain, Mansoor; Hui, Chi-Chung; Backx, Peter H.^*

来源：American Journal of Physiology - Heart and Circulatory Physiology, 2022, 322(5): H725-H741.

DOI：10.1152/ajpheart.00572.2021

• contractility
• Irx5
• I-to
• transmural gradients

Previous studies have established that transmural gradients of the fast transient outward K+ current (I-to,I-f) correlate with regional differences in action potential (AP) profile and excitation-contraction coupling (ECC) with high I-to,I-f expression in the epimyocardium (EPI) being associated with short APs and low contractility and vice versa. Herein, we investigated the effects of altering the I-to,I-f gradients on transmural contractile properties using mice lacking Irx5 (Irx5-KO) or lacking Kcnd2 (K-V.2-KO) or both. Irx5-KO mice exhibited decreased global LV contractility in association with elevated Ito,f, as well as reduced cell shortening and Ca2+ transient amplitudes in cardiomyocytes isolated from the endomyocardium (ENDO) but not in cardiomyocytes from the EPI. Transcriptional profiling revealed that the primary effect of Irx5 ablation on ECC-related genes was to increase I-to,I-f gene expression (i.e., Kcnd2 and Kcnip2) in the ENDO, but not the EPI. By contrast, K(V)4.2-KO mice showed selective increases in cell shortening and Ca2+ transients in isolated EPI cardiomyocytes, leading to enhanced ventricular contractility and mice lacking both Irx5 and Kcnd2 displayed elevated ventricular contractility, comparable to K(V)4.2-KO mice, demonstrating a dominant role of Irx5-dependent modulation of Ito,f in the regulation of contractility. Our findings show that the transmural electromechanical heterogeneities in the healthy ventricles depend on the Irx5-dependent I-to,I-f gradients. These observations provide a useful framework for assessing the molecular mechanisms underlying the alterations in contractile heterogeneity seen in the diseased heart. @@@ NEW & NOTEWORTHY Irx5 is a vital transcription factor that establishes the transmural heterogeneity of ventricular myocyte contractility, thereby ensuring proper contractile function in the healthy heart. Regional differences in excitation-contraction coupling in the ventricular myocardium are primarily mediated through the inverse relationship between Irx5 and the fast transient outward K+ current (I-to,I-f) across the ventricular wall.