Overexpression of megsin induces mesangial cell proliferation and excretion of type IV collagen in vitro

作者：Xia Yunfeng; Zhang Yimin; Shi Wei^*; Liu Shuangxin; Chen Yuanhan; Liang Xinling; Ye Zhiming

来源：Cellular Immunology, 2011, 271(2): 413-417.

DOI：10.1016/j.cellimm.2011.08.009

• Megsin
• Transfection
• Mesangial cell
• Proliferation
• Cytokine

Over-expression of megsin is associated with mesangial cell (MC) proliferation and extracellular matrix (ECM) accumulation. The underlying pathogenesis is unknown. This study demonstrate that overexpression of megsin induced incorporation of [H-3]thymidine in MCs and PDGF-BB, TGF-beta 1 upregulation. Concentrations of PDGF-BB, TGF-beta 1 and type IV collagen in the culture medium of MCs transfected with megsin were higher than controls. Anti-PDGF-BB suppressed incorporation of [H-3]thymidine in MCs transfected with megsin and mRNA expression of TGF-beta 1 in stable transformant MCs, suggesting that over-expression of megsin induces cell proliferation and ECM accumulation in MCs, upregulation of PDGF-BB and TGF-beta 1 is probably the main route involved in pathogenesis.

• 单位
  广东省人民医院