Epidemiological and evolutionary characteristics of the PRRSV in Southern China from 2010 to 2013

作者：Xie Jiexiong; Cui Tiantian; Cui Jin; Chen Yao; Zhang Minze; Zhou Pei; Deng Shengchao; Su Shuo^*; Zhang Guihong

来源：Microbial Pathogenesis, 2014, 75: 7-15.

DOI：10.1016/j.micpath.2014.08.001

• Porcine reproductive and respiratory syndrome virus (PRRSV)
• GP5
• Nsp2
• Molecular epidemiology
• South China

In 2006, a highly pathogenic strain of porcine reproductive and respiratory syndrome virus (HP-PRRSV) emerged in China and caused lasting damage to the swine industry. To analyze the genetic variation of PRRSV in Southern China, 126 tissue samples were collected; 41 ORF5 and partial Nsp2 genes were sequenced and analyzed. The results showed that the PRRSV positive rate was 32.54% over the last four years, that there are two main subgenotypes in Southern China, and that the dominant strain is HP-PRRSV. An amino acid analysis of Nsp2 showed that 40 strains contained a 30-amino acid deletion in the hypervariable region. However, the 13YJ6-8 mutant exhibited a unique amino acid deletion at positions 508-514 of Nsp2. A phylogenetic analysis of ORF5 revealed that this mutant and five other strains, belong to an intermediate subgenotype (inter-subgenotype), which is characterized by extensive mutations, especially in the signal peptide and N-glycosylation sites. The results of this study demonstrate the genetic diversity of PRRSV in Southern China and provide basic knowledge of the PRRSV epidemic in this region.

• 单位
  华南农业大学