Electrochemical DNA detection using Hoechst dyes in microfluidic chips

作者：Zhao H; Li Z; Lee N Y; Kim J S; Lee E C^*

来源：Current Applied Physics, 2012, 12(6): 1493-1496.

DOI：10.1016/j.cap.2012.04.023

• DNA sensor
• Hoechst
• Immobilization-free process
• Microfluidic chip
• Regeneration

We fabricate microfluidic DNA sensors based on the electrochemical method that uses Hoechst 33258 [2′-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)- 2,5′-bi(1H-benzimidazole)] as a DNA intercalator. These chips are fabricated without a probe immobilization process, thereby being more simply regenerated than other typical DNA chips. Linear sweep voltammetric measurements show an anodic-current decrease consistent with the DNA duplex formation which occurs when the target is fully complementary to the probe, enabling sequence-specific DNA detection.