Ectopic B7-H4-Ig expression attenuates concanavalin A-induced hepatic injury

作者：Xu Jun Fa^*; Xiao Huan; Hu Guo Yan; Zheng Shu Hua; Liu Wei; Yuan Chun Lei; Yang Heng; Lue Jing; Zheng Fang; Wang Cong Yi; Gong Fei Li

来源：Clinical Immunology, 2010, 136(1): 30-41.

DOI：10.1016/j.clim.2010.02.022

• B7-H4
• concanavalin A
• hydrodynamics-based gene delivery
• hepatic injury
• IL-2
• IFN-gamma
• IL-4
• IL-10

Previous studies demonstrate that both membrane B7-H4 and B7-H4-Ig fusion protein could inhibit T-cell responses. In the present study, we explored the potential effect of B7-H4-Ig on liver injury in a hepatitis mouse model induced by concanavalin A (ConA). A B7-H4-Ig construct was introduced into animals by the hydrodynamic gene delivery approach. It was found that ectopic expression of B7-H4-Ig could inhibit ConA-induced elevation of serum levels of ALT and AST, suppress liver necrosis and even mortality of mice. Furthermore, we observed that pretreatment of B7-H4-Ig dramatically decreased serum levels and the expression of mRNA for IL-2, IFN-gamma and IL-4, but increased IL-10 in ConA-treated mice. Our results suggest that B7-H4-Ig may protect animals from liver injury induced by ConA, which could be associated with reduced serum levels for IL-2, IFN-gamma and IL-4 as well as enhanced IL-10 production.

• 单位
  广东医学院