A zwitterionic 1D/2D polymer co-crystal and its polymorphic sub-components: a highly selective sensing platform for HIV ds-DNA sequences

作者：Zhao Hai Qing; Yang Shui Ping; Ding Ni Ni; Qin Liang; Qiu Gui Hua; Chen Jin Xiang^*; Zhang Wen Hua^*; Chen Wen Hua^*; Hor T S Andy^*

来源：Dalton Transactions, 2016, 45(12): 5092-5100.

DOI：10.1039/c5dt04410c

Polymorphic compounds {[Cu(dcbb)(2)(H2O)(2)]center dot 10H(2)O}(n) (2, 1D chain), [Cu(dcbb) 2] n (3, 2D layer) and their co-crystal {[Cu(dcbb)(2)(H2O)][Cu(dcbb)(2)](2)}(n) (4) have been prepared from the coordination reaction of a 2D polymer [Na(dcbb)(H2O)](n) (1, H2dcbbBr = 1-(3,5-dicarboxybenzyl)-4,4'-bipyridinium bromide) with Cu(NO3)(2)center dot 3H(2)O at different temperatures in water. Compounds 2-4 have an identical metal-to-ligand stoichiometric ratio of 1 : 2, but absolutely differ in structure. Compound 3 features a 2D layer structure with aromatic rings, positively charged pyridinium and free carboxylates on its surface, promoting electrostatic, pi-stacking and/or hydrogen-bonding interactions with the carboxyfluorescein (FAM) labeled probe single-stranded DNA (probe ss-DNA, delineates as P-DNA). The resultant P-DNA@3 system facilitated fluorescence quenching of FAM via a photoinduced electron transfer process. The P-DNA@3 system functions as an efficient fluorescent sensor selective for HIV double-stranded DNA (HIV ds-DNA) due to the formation of a rigid triplex structure with the recovery of FAM fluorescence. The system reported herein also distinguishes complementary HIV ds-DNA from mismatched target DNA sequences with the detection limit of 1.42 nM.

• 单位
  南方医科大学; 苏州大学