我是社会性信息

作者：Huang Jiahao; Su Xuefen; Li Zhigang^*

来源：ANALYTICAL CHEMISTRY, 2012, 84(14).

DOI：10.1021/ac3004727

• 大数据测试20190725
• 0725
• 测试072901
• 子集关键词
• 关键词测试0729

In this work, we propose a simple and enzyme-free strategy for sensitive and selective DNA detection by using two different types of molecular beacons (MBs), MB1 and MB2. In this method, the target DNA binds with and restores the fluorescence of MB1 first. Then, MB2 hybridizes with MB1 and free the target, which is used to trigger another reaction cycle. The cycling use of the target and the employment of bi-MBs amplify the fluorescence intensity for sensitive DNA detection. The detection limit of this method was obtained as 10 pM, which is about 2 orders of magnitude sensitive than the conventional MB-based approaches.

• 单位
  1