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摘要
The H240R protein (pH240R), encoded by the H240R gene of African swine fever virus (ASFV), is a 241-amino-acid capsid protein. We previously showed that the deletion of H240R from the ASFV genome, creating ASFV-Delta H240R, resulted in an approximately 2-log decrease in infectious virus production compared with the wild-type ASFV strain (ASFV-WT), and ASFV-Delta H240R induced higher interleukin 1 beta (IL-1 beta) production in porcine alveolar macrophages (PAMs) than did ASFV-WT, but the underlying mechanism remains to be elucidated. Here, we demonstrate that the activation of the NF-kappa B signaling and NLRP3 inflammasome was markedly induced in PAMs upon ASFV-AH240R infection compared with ASFV-WT. Moreover, pH240R inhibited NF-kappa B activation by interacting with NEMO and promoting the autophagy-mediated lysosomal degradation of NEMO, resulting in reduced pro-IL-1 transcription. Strikingly, NLRP3 deficiency in PAMs inhibited the ASFV-AH240R-induced IL-1 beta secretion and caspase 1 activation, indicating an essential role of NLRP3 inflammasome activation during ASFV-AH240R replication. Mechanistically, pH240R interacted with NLRP3 to inhibit its oligomerization, leading to decreased IL-1 beta production. Furthermore, the inhibition of the NF-kappa B signaling and NLRP3 inflammasome activation promoted ASFV-AH240R replication in PAMs. Taken together, the results of this study reveal an antagonistic mechanism by which pH240R suppresses the host immune response by manipulating activation of the NF-kappa B signaling and NLRP3 inflammasome, which might guide the rational design of live attenuated vaccines or therapeutic strategies against ASF in the future. @@@ IMPORTANCE African swine fever (ASF), a lethal hemorrhagic disease, is caused by African swine fever virus (ASFV). There are no commercially available vaccines or antivirals for the disease. Here, we showed that ASFV with a deletion of the H240R gene exhibits high-level expression of interleukin 1 beta (IL-1 beta), a proinflammatory cytokine, in porcine alveolar macrophages and that the H240R protein (pH240R) exhibits robust inhibitory effects on IL-1# transcription and production. More specifically, pH240R inhibited NF-kappa B activation via the autophagy-mediated lysosomal degradation of NEMO, leading to the decrease of pro-IL-10 transcription. In addition, pH240R interacted with NLRP3 to inhibit its oligomerization, leading to decreased IL-1 beta production. Our results indicate that pH240R is involved in the evasion of host innate immunity and provide a novel target for the development of a live attenuated vaccine against ASF.
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单位y; 哈尔滨医科大学; 东北农业大学
