Comprehensive analysis of transcriptome-wide M6A methylation for hepatic ischaemia reperfusion injury in mice

作者：Hua, Yongliang; Li, Xinglong; Yin, Bing; Huang, Jingjing; Lu, Shounan; Wang, Chaoqun; Ke, Shanjia; Xu, Yanan; Qian, Baolin; Feng, Zhigang; Yu, Hongjun; Ma, Yong^*

来源：Epigenetics, 2023, 18(1): 2201716.

DOI：10.1080/15592294.2023.2201716

• N6-methyladenosine
• hepatic ischaemia reperfusion injury
• MeRIP-seq
• posttranscriptional regulation
• methyltransferase

N6-Methyladenosine (m(6)A) plays key roles in the regulation of biological functions and cellular mechanisms for ischaemia reperfusion (IR) injury in different organs. However, little is known about the underlying mechanisms of m(6)A-modified mRNAs in hepatic IR injury. In mouse models, liver samples were subjected to methylated RNA immunoprecipitation with high-throughput sequencing (MeRIP-seq) and RNA sequencing (RNA-seq). In total, 16917 m(6)A peaks associated with 4098 genes were detected in the sham group, whereas 21,557 m(6)A peaks associated with 5322 genes were detected in the IR group. There were 909 differentially expressed m(6)A peaks, 863 differentially methylated transcripts and 516 differentially m(6)A modification genes determined in both groups. The distribution of m(6)A peaks was especially enriched in the coding sequence and 3'UTR. Furthermore, we identified a relationship between differentially m(6)A methylated genes (fold change >= 1.5/<= 0.667, p value <= 0.05) and differentially expressed genes (fold change >= 1.5 and p value <= 0.05) to obtain three overlapping predicted target genes (Fnip2, Phldb2, and Pcf11). Our study revealed a transcriptome-wide map of m(6)A mRNAs in hepatic IR injury and might provide a theoretical basis for future research in terms of molecular mechanisms.

• 单位
  1; 哈尔滨医科大学; y