A 33-color panel of phenotypic analysis of murine organ specific immune cells

作者：Yang, Si-Yu; Huang, Meng-Xing; Sun, Yan-Xia; Li, Liang; Bian, Zhen-Hua; Long, Jie; Zhao, Zhi-Bin^*

来源：Journal of Immunological Methods, 2022, 507: 113294.

DOI：10.1016/j.jim.2022.113294

• 33-color panel
• Phenotypic analysis
• Organ specific immune cells
• Flow cytometry

This 33-color panel delineates immune phenotypes of the major lymphocyte subsets in murine tissues. The panel identifies surface markers for cell activation, differentiation, and exhaustion, and chemokine receptors, in CD4 T cells, CD8 T cells, gamma delta T cells, NK cells, NKT cells and B cells in spleen and liver. This panel was designed to include only surface markers to avoid the need for fixation and permeabilization steps. Cells were analyzed on a full spectrum flow cytometer, 5-Laser Aurora system (Cytek Biosciences). This panel enables in-depth immunophenotyping of murine lymphocytes in immune and non-immune tissues of mice. It could be a tool for systematic analysis of immune cell response to infectious diseases and immune disorders.

• 单位
  广东省人民医院; 广东省心血管病研究所