In vivo multifunctional fluorescence imaging using liposome-coated lanthanide nanoparticles in near-infrared-II/IIa/IIb windows

作者：Yang, Junying; He, Shuqing; Hu, Zhenhua^*; Zhang, Zeyu; Cao, Caiguang; Cheng, Zhen^*; Fang, Chihua^*; Tian, Jie^*

来源：Nano Today, 2021, 38: 101120.

DOI：10.1016/j.nantod.2021.101120

• NIR-II
• IIa
• IIb windows
• Fluorescence imaging
• Brown adipose tissue
• Multifunctional nanoprobe
• Rare-earth-doped nanoparticle
• Excretory capacity

In vivo fluorescence imaging in the near-infrared II window (NIR-II, 1000-1700 nm) has great improvements in imaging quality compared with the visible (400-700 nm) and the NIR-I (700-900 nm) window due to the low autofluorescence and the reduced scattering. To date, a variety of NIR-II fluorophores have been synthesized and applied in vivo imaging and disease diagnosis. Multifunction is believed as one of the developing trends. In this study, we report a liposome-coated lanthanide nanoparticle, named as NPs@Lips, which can emit three-wavelengths fluorescence (1000-1100 nm (NIR-II), 1300-1350 nm (NIR-IIa), 1500-1700 nm (NIR-IIb) simultaneously under an 808 nm laser excitation. The imaging results demonstrated that the images in longer wavelengths provided a higher resolution and enhanced signal-to-noise (S/N) ratio than the ones in shorter wavelengths. Then, the NPs@Lips were studied for in vivo multifunctional biological imaging, including brown adipose tissue imaging, vascular imaging and lymph node localization biopsy. Interestingly, NPs@Lips can accumulate in brown adipose tissue without any targeting molecules, which provided an alternative sensitive and non-radiation method for brown adipose tissue imaging in the field of metabolic disorders. Overall, the NPs@Lips were potential NIR-II probe for multifunctional nanomedicine applications.

• 单位
  中国科学院; 南方医科大学; 北京航空航天大学; 中国科学院研究生院